{"id":402610,"date":"2024-10-20T05:02:01","date_gmt":"2024-10-20T05:02:01","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-cen-ts-177182022\/"},"modified":"2024-10-26T08:57:46","modified_gmt":"2024-10-26T08:57:46","slug":"bsi-pd-cen-ts-177182022","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-cen-ts-177182022\/","title":{"rendered":"BSI PD CEN\/TS 17718:2022"},"content":{"rendered":"

This document provides the methodology for the enumeration and determination of Rhizobium sp., Mesorhizobium sp., Ensifer sp., or Bradyrhizobium sp. in plant biostimulant products in accordance with the Regulation (EU) 2019\/1009 of the European Parliament and of the Council [1].<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
5<\/td>\nEuropean foreword <\/td>\n<\/tr>\n
6<\/td>\nIntroduction <\/td>\n<\/tr>\n
7<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
8<\/td>\n4 Enumeration of Rhizobium sp., Mesorhizobium sp., Ensifer sp., Bradyrhizobium sp.
4.1 General
4.2 Sample preparation
4.2.1 General
4.2.2 Liquid \u2013 water-based formulations
4.2.3 Liquid \u2013 oil-based, emulsifiable concentrate (EC) formulations <\/td>\n<\/tr>\n
9<\/td>\n4.2.4 Solid \u2013 wettable powder (WP) formulations
4.2.5 Solid \u2013 water dispersible granules (WDG) formulations
4.2.6 Solid \u2013 pellets, granules, microgranules – slow release – formulations
4.2.7 Solid \u2013 substrate
4.3 Serial dilution <\/td>\n<\/tr>\n
10<\/td>\n4.4 Plate counts of rhizobia in sterile diluent
4.5 Spread-plate counting <\/td>\n<\/tr>\n
11<\/td>\n4.6 Growth Media
4.7 Calculation
5 Species determination of Rhizobium sp., Mesorhizobium sp., Ensifer sp., Bradyrhizobium sp. via genetic analysis
5.1 General
5.2 Preparation of the sample for the genomic DNA extraction
5.2.1 Isolation and preparation of the microorganism <\/td>\n<\/tr>\n
12<\/td>\n5.2.2 Sample concentration
5.2.3 DNA extraction and storage
5.2.4 Partial PCR Amplification of the 16S rRNA Genes <\/td>\n<\/tr>\n
14<\/td>\nAnnex A (informative) Formula of culture media <\/td>\n<\/tr>\n
17<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Plant biostimulants. Determination of Rhizobium spp.<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2022<\/td>\n18<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":402618,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-402610","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/402610","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/402618"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=402610"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=402610"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=402610"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}