{"id":341905,"date":"2024-10-19T23:58:54","date_gmt":"2024-10-19T23:58:54","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-iso-ts-21872-22020\/"},"modified":"2024-10-25T23:12:38","modified_gmt":"2024-10-25T23:12:38","slug":"bsi-pd-iso-ts-21872-22020","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-iso-ts-21872-22020\/","title":{"rendered":"BSI PD ISO\/TS 21872-2:2020"},"content":{"rendered":"<p>This document specifies a method for the direct enumeration of potentially enteropathogenic <i>V. parahaemolyticus<\/i> (<i>tdh<\/i> and\/or <i>trh<\/i> positive) and\/or the enumeration of total <i>V. parahaemolyticus<\/i> in seafood.<\/p>\n<h4>PDF Catalog<\/h4>\n<table border=\"1px\" class=\"des-table\">\n<tr>\n<th>PDF Pages<\/th>\n<th>PDF Title<\/th>\n<\/tr>\n<tr>\n<td><b>2<b><\/td>\n<td>undefined  <\/td>\n<\/tr>\n<tr>\n<td><b>6<b><\/td>\n<td>Foreword  <\/td>\n<\/tr>\n<tr>\n<td><b>7<b><\/td>\n<td>Introduction  <\/td>\n<\/tr>\n<tr>\n<td><b>9<b><\/td>\n<td>1 Scope <br \/> 2 Normative references <br \/> 3 Terms and definitions  <\/td>\n<\/tr>\n<tr>\n<td><b>10<b><\/td>\n<td>4 Principle <br \/> 4.1 General <br \/> 4.2 Plating <br \/> 4.3 Preparation of membranes <br \/> 4.4 Hybridization <br \/> 4.5 Detection  <\/td>\n<\/tr>\n<tr>\n<td><b>11<b><\/td>\n<td>5 Culture media and reagents <br \/> 5.1 General <br \/> 5.2 Hybridization reagents <br \/> 5.2.1 Hybridization buffer <br \/> 5.2.2 Digoxigenin-labelled oligoprobes <br \/> 5.2.3 Control materials <br \/> 6 Equipment and consumables  <\/td>\n<\/tr>\n<tr>\n<td><b>12<b><\/td>\n<td>7 Sampling <br \/> 8 Procedure <br \/> 8.1 General <br \/> 8.2 Preparation of the sample <br \/> 8.3 Plating <br \/> 8.4 Preparation of process control <br \/> 8.5 Preparation of membranes <br \/> 8.5.1 Colony lift  <\/td>\n<\/tr>\n<tr>\n<td><b>13<b><\/td>\n<td>8.5.2 Cross-linking of DNA to the membrane <br \/> 8.5.3 Proteinase K treatment <br \/> 8.6 Hybridization <br \/> 8.6.1 General  <\/td>\n<\/tr>\n<tr>\n<td><b>14<b><\/td>\n<td>8.6.2 Hybridization of tdh and trh probes <br \/> 8.6.3 Colorimetric detection  <\/td>\n<\/tr>\n<tr>\n<td><b>15<b><\/td>\n<td>8.6.4 Enumeration of tdh and\/or trh positive colonies <br \/> 8.6.5 Isolation of colonies (optional) <br \/> 8.6.6 Hybridization of toxR probe  <\/td>\n<\/tr>\n<tr>\n<td><b>16<b><\/td>\n<td>8.6.7 Colorimetric detection <br \/> 8.6.8 Enumeration of toxR positive colonies <br \/> 9 Interpretation of the results  <\/td>\n<\/tr>\n<tr>\n<td><b>17<b><\/td>\n<td>10 Expression of results <br \/> 11 Test report  <\/td>\n<\/tr>\n<tr>\n<td><b>18<b><\/td>\n<td>Annex A (normative)  Flow diagram for the enumeration of total and potentially enteropathogenic Vibrio parahaemolyticus in seafood using nucleic acid hybridization  <\/td>\n<\/tr>\n<tr>\n<td><b>21<b><\/td>\n<td>Annex B (normative)  Culture media and reagents  <\/td>\n<\/tr>\n<tr>\n<td><b>27<b><\/td>\n<td>Annex C (informative)  Hybridization of tdh, trh and toxR genes of V. parahaemolyticus  <\/td>\n<\/tr>\n<tr>\n<td><b>35<b><\/td>\n<td>Bibliography  <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p><b>Microbiology of the food chain. Horizontal method for the determination of <i>Vibrio<\/i> spp. &#8211; Enumeration of total and potentially enteropathogenic <i>Vibrio parahaemolyticus<\/i> in seafood using nucleic acid hybridization<\/b><\/p>\n<table class=\"shortdes-table\" style=\"width: 100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Published By<\/td>\n<td>Publication Date<\/td>\n<td>Number of Pages<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/pdfstandards.shop\/product-category\/publishers\/bsi\/\"><b>BSI<\/b><\/a><\/td>\n<td>2020<\/td>\n<td>36<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":341913,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-341905","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/341905","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/341913"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=341905"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=341905"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=341905"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}