{"id":338423,"date":"2024-10-19T23:40:52","date_gmt":"2024-10-19T23:40:52","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-214462019\/"},"modified":"2024-10-25T22:46:59","modified_gmt":"2024-10-25T22:46:59","slug":"bs-iso-214462019","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-214462019\/","title":{"rendered":"BS ISO 21446:2019"},"content":{"rendered":"

This document specifies a method for the quantitative determination of trans<\/i> and total (cis<\/i> + trans<\/i>) vitamin K1<\/sub> in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 \u03bcg\/l to 80 \u03bcg\/l trans<\/i> vitamin K1<\/sub>, and the limit of quantification (LOQ) was estimated to be 0,4 \u03bcg\/l for standards and 0,09 \u03bcg\/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
7<\/td>\n1 Scope
2 Normative references
3 Terms and definitions
4 Principle <\/td>\n<\/tr>\n
8<\/td>\n5 Reagents and materials
6 Standard and solution preparation
6.1 Mixture with volume fraction of 10 % isopropanol in iso-octane
6.2 Mobile phase <\/td>\n<\/tr>\n
9<\/td>\n6.3 Post-column electrolyte solution
6.4 Vitamin K1 (phytonadione) stock standard solution
6.4.1 Vitamin K1 (phytonadione) stock standard solution
6.4.2 Stock standard concentration (for non USP standard)
6.5 Vitamin K1 (phytonadione) intermediate I standard solution
6.6 Vitamin K1 (phytonadione) intermediate II standard solution
6.7 Vitamin K1 (phytonadione) working standard solutions <\/td>\n<\/tr>\n
10<\/td>\n7 Apparatus
8 Procedure
8.1 Sample preparation
8.1.1 Liquid samples <\/td>\n<\/tr>\n
11<\/td>\n8.1.2 Powder samples
8.1.3 Dry blended\/non-homogenous powder samples
8.1.4 Wet blended powder samples
8.1.5 Extraction <\/td>\n<\/tr>\n
12<\/td>\n8.2 HPLC analysis
8.2.1 Instrumental operating conditions
8.2.2 Instrument start-up <\/td>\n<\/tr>\n
13<\/td>\n8.3 HPLC of standards and samples <\/td>\n<\/tr>\n
14<\/td>\n8.4 Instrument shut-down
8.4.1 Short-term shut-down
8.4.2 Long-term shut-down
9 Calculations <\/td>\n<\/tr>\n
16<\/td>\n10 Precision
10.1 General
10.2 Repeatability
10.3 Reproducibility <\/td>\n<\/tr>\n
18<\/td>\nAnnex A (informative) Example chromatograms <\/td>\n<\/tr>\n
20<\/td>\nAnnex B (informative) Precision data <\/td>\n<\/tr>\n
24<\/td>\nAnnex C (informative) Comparison between this document and EN 14148 <\/td>\n<\/tr>\n
26<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Infant formula and adult nutritionals. Determination of trans<\/i> and total (cis<\/i> + trans<\/i>) vitamin K1<\/sub> content. Normal phase HPLC<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2019<\/td>\n28<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338427,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-338423","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338423","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338427"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338423"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338423"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338423"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}