BSI PD CEN/TR 16193:2013

$198.66

Sludge, treated biowaste and soil. Detection and enumeration of Escherichia coli

Published By	Publication Date	Number of Pages
BSI	2013	56

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Categories: 13.030.01 - Wastes in general, BSI

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PDF Catalog

PDF Pages	PDF Title
8	1 Scope 2 Normative references 3 Terms and definitions
9	4 Abbreviations 5 Quality assurance 6 Method A — Membrane filtration method for quantification 6.1 Scope
10	6.2 Principle 6.3 Reagents, diluents and culture media
11	6.4 Apparatus
12	6.5 Sampling 6.5.1 General
13	6.5.2 Storage 6.5.3 Handling 6.6 Procedure 6.6.1 Sample preparation 6.6.1.1 General 6.6.1.2 For lime-treated materials 6.6.1.3 Centrifugation (optional) 6.6.1.4 Pre-filtration (optional)
14	6.6.2 Sample dilution 6.6.3 Membrane filtration
15	6.6.4 Resuscitation and enumeration of colonies on chromogenic agar 6.6.5 Confirmation of colony identity 6.6.6 Determination of the dry residue content 6.7 Calculation and expression of results
16	6.8 Performance data of the interlaboratory comparison — Method A 6.8.1 Material used in the interlaboratory comparison study
17	6.8.2 First assessment of the precision of the method
18	6.8.3 Interlaboratory comparison results
19	6.9 Pre-filtration and centrifugation — Comparison tests
21	7 Method B — Miniaturised method (Most Probable Number) by inoculation in liquid medium 7.1 Scope 7.2 Principle 7.3 Reagents, diluents and culture media
23	7.4 Apparatus
24	7.5 Sampling 7.5.1 General 7.5.2 Storage 7.5.3 Handling 7.6 Procedure 7.6.1 Sample preparation 7.6.1.1 General
25	7.6.1.2 For lime-treated materials 7.6.2 Analysis 7.6.2.1 Preparation of dilutions 7.6.2.2 Inoculation and incubation of microtitre plates
26	7.6.2.3 Reading 7.6.3 Determination of the dry residue content 7.7 Expression of results 7.7.1 Determination of the characteristic number
27	7.7.2 Calculation of the MPN and its confidence interval
29	7.8 Performance data 7.8.1 MPN Statistical table
37	7.8.2 Performance data of the interlaboratory comparison 7.8.2.1 Matrices — Method B
38	7.8.3 First assessment of the precision of the method
39	7.8.4 Interlaboratory comparison results
41	7.9 Preparation of synthetic sea salt 7.9.1 Major ion composition of a convenient ocean synthetic sea salt 7.9.2 Example for preparation from defined substances
42	7.10 Quality criteria for the manufacturing of the medium in microtitre plates (E. coli)
43	8 Method C — Macromethod (Most Probable Number) in liquid medium 8.1 Scope 8.2 Principle 8.3 Reagents, diluents and culture media
44	8.4 Apparatus
45	8.5 Sampling 8.5.1 General 8.5.2 Sample storage 8.5.3 Sample handling 8.6 Procedure 8.6.1 Sample preparation
46	8.6.2 Analysis 8.6.3 Determination of the dry residue content 8.7 Expression of the results
48	8.8 Performance data 8.8.1 MPN Statistical table for 3-tubes MPN procedure
49	8.8.2 Repeatability and reproducibility 8.8.2.1 Matrices — Method C 8.8.3 First assessment of the precision of the method
50	8.8.4 Interlaboratory comparison results
52	9 Test report