🔥🔥 Don’t Miss Out on Our End of Autumn Sale. If you have any questions, feel free to click the bottom right corner to contact our customer service..

Concat us[email protected]
Shopping Cart

No products in the cart.

🔍
BSI 23/30451046 DC:2023 Edition

BSI 23/30451046 DC:2023 Edition

$13.70

BS EN ISO 19045-2. Ophthalmic optics. Contact lens care products – Part 2. Method for evaluating disinfecting efficacy by contact lens care products using trophozoites of Acanthamoeba species as the challenge organisms

Published By Publication Date Number of Pages
BSI 2023 33
PDF Format Searchable Printable Preview Now
Guaranteed Safe Checkout
Category:

If you have any questions, feel free to reach out to our online customer service team by clicking on the bottom right corner. We’re here to assist you 24/7.
Email:[email protected]

PDF Catalog

PDF Pages PDF Title
6 Foreword
7 1 Scope
2 Normative references
3 Terms and definitions
8 4 Principle
4.1 General
5 Acanthamoeba trophozoite disinfecting test method
5.1 Organisms
5.2 Culture media and reagents
9 5.3 Test materials
5.4 Test samples
5.5 Culture maintenance
10 5.6 Growth and harvest of microbial challenge (trophozoite)
5.7 Preparation of Acanthamoeba stock solution
5.8 Stand-alone procedure – inoculation
11 5.9 Recovery Procedures
5.9.1 Stand-alone procedure – recovery method one (12 well plate method)
5.9.2 Stand-alone procedure – recovery method two (96 well plate method)
13 6 Controls
6.1 Inoculum Control
14 6.2 Recovery medium control
7 Performance Criteria
15 Annex€A (normative) Preparation of Acanthamoeba growth medium (Ac#6)
16 Annex€B (normative) Preparation of ¼ Strength Ringer’s Solution
17 Annex€C (informative) Maintenance of Acanthamoeba trophozoites and preparation for testing
18 Annex€D (normative) Preparation of Non-Nutrient Agar (NNA)
20 Annex€E (informative) Preparation of E.coli Suspension
21 Annex€F (informative) Preparation of Non-Nutrient Agar (NNA) Plates with E.coli
22 Annex€G (informative) Preparation of Neutraliser Broth for recovery methods
23 Annex€H (normative) Reed and Muench Computation Method for Calculation of the 50€% Endpoint Titre
27 Annex€I (normative) Spearman-Karber Computation Method for Calculation of the 50€% Endpoint Titre
29 Annex€J (normative) Micrographs of Cultures Using the 96 Well Plate Method
33 Bibliography

Related products

BSI 23/30451046 DC
$13.70