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BSI 22/30450670 DC 2022

BSI 22/30450670 DC 2022

$13.70

BS ISO 7581. Method for the evaluation of basic bactericidal activity of a non-porous surface

Published By Publication Date Number of Pages
BSI 2022 31
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PDF Catalog

PDF Pages PDF Title
1 30450670-NC.pdf
3 ISO_DIS 7581 ed.1 – id.83386 Enquiry PDF (en).pdf
6 Foreword
7 Introduction
9 1 Scope
2 Normative reference
3 Terms and definitions
11 4 Materials and reagents
4.1 Test organisms
4.2 Culture media and reagents
4.2.1 General
4.2.2 Water
4.2.3 Microbial suspension diluents
4.2.4 Liquid for bacteria recovery and for membrane rinsing
12 4.2.5 Agar for bacteria maintenance and counting (tryptone soya agar: TSA)
4.3 Test surfaces
4.3.1 Reference surfaces
4.3.2 Test surfaces
13 4.4 Apparatus and glassware
4.4.1 General
4.4.2 Usual microbiological laboratory equipment, and in particular:
15 5 Preparation of the test organism suspensions
5.1 Stock cultures of the test bacteria
5.2 Working culture of the test bacteria
5.3 Test suspensions
5.4 Counting bacterial test suspensions
16 5.5 Calculation of the weighted mean values of the test suspensions
17 6 Evaluation method of the bactericidal activity of a non-porous surface and its efficacy
6.1 Compulsory experimental conditions
6.2 Test procedure
6.2.1 Sampling
6.2.2 The test itself (E0 and E1h)
18 6.2.3 Control reference surfaces (C0 and C1h)
6.2.4 Validation (Vn)
19 7 Expression of results
7.1 Determination of the number of viable bacteria
20 7.2 Verification of the methodology
7.3 Expression of results
7.3.1 Reduction
21 7.3.2 Conclusion: The bactericidal activity of non-porous surfaces
8 Test report
23 Annex€A (normative) Corresponding reference strains
24 Annex€B (informative) Standard test report of the measurement of the bactericidal activity of non-porous surfaces
26 Annex€C (informative) Examples of surface preparation protocols
27 Annex€D (informative) Neutralizers
29 Annex€E (informative) Technical guide to depositing the inoculum
31 Bibliography

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BSI 22/30450670 DC 2022
$13.70