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BS ISO 7102:2024

$142.49

Infant formula. Determination of β-galactooligosaccharides. Ultra high performance liquid chromatography (UHPLC) with fluorescence detection after pre-column derivatization

Published By Publication Date Number of Pages
BSI 2024 28
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PDF Catalog

PDF Pages PDF Title
2 undefined
6 Forewords
9 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
10 5 Chemicals and reagents
5.1 List of chemicals and reagents
11 5.2 Preparation of reagents
12 5.3 Preparation of mobile phases
5.4 Preparation of standard solutions
6 Apparatus
13 7 Procedure
7.1 Sample preparation
7.1.1 Powdered or concentrated products on a ready-to feed (RTF) basis
14 7.1.2 Reconstituted products as prepared in 7.1.1 or products sold as RTF
7.1.3 Homogeneous powdered products without prior reconstitution
7.1.4 Calibration curve
7.1.5 Dextran ladder
7.1.6 Hydrolysis of maltodextrins and GOS
7.1.7 Reagent blank
7.1.8 Internal standard addition
7.1.9 Derivatization
15 7.1.10 Dilution
7.2 Chromatographic conditions
7.3 System suitability test
7.4 Calibration
16 8 Calculation
9 Precision data
9.1 General
17 9.2 Repeatability
9.3 Reproducibility
10 Test report
18 Annex A (normative) Assessment of maltotriose concentration
20 Annex B (informative) Determination of molecular weight using dextran ladder
21 Annex C (informative) Determination of molecular weight using LC-MS
23 Annex D (informative) Example chromatograms
25 Annex E (informative) Precision data
27 Bibliography
BS ISO 7102:2024
$142.49