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BS ISO 18074:2015

BS ISO 18074:2015

$167.15

Textiles. Identification of some animal fibres by DNA analysis method. Cashmere, wool, yak and their blends

Published By Publication Date Number of Pages
BSI 2015 34
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This International Standard specifies a testing method for DNA analysis of some animal fibres to identify cashmere, wool, yak, and their blends by using extraction, amplification by the polymerase chain reaction (PCR) method and DNA detection processes.

This International Standard is applicable to cashmere, yak, and wool and their blends as a qualitative method.

PDF Catalog

PDF Pages PDF Title
6 Foreword
7 Introduction
9 1 Scope
2 Caution
3 Normative references
4 Terms and definitions
10 5 Principle
11 6 Apparatus and equipment
12 7 Reagents
14 8 Sampling
9 Test methods
9.1 General
9.2 Chipping sample
9.3 DNA extraction
15 9.4 DNA purification
16 9.5 DNA amplification
9.5.1 Composition of the reaction solution
9.5.2 Condition of PCR amplification instrument for DNA amplification
9.5.3 DNA Amplification test method
17 9.6 Detection and confirmation of DNA amplification
9.6.1 Preparation
9.6.2 Electrophoretic migration test
10 Verification
10.1 General
10.2 No amplification verification after extraction process (negative verification)
18 10.3 Amplification verification after extraction process (positive verification)
10.4 Amplification verification after PCR process
10.5 No amplification verification after PCR process
10.6 Amplification verification of PCR reaction solution
19 11 Assessment
12 Precision
13 Test report
21 Annex A (informative) Amplification of the constant length DNA fragment by PCR method
23 Annex B (informative) DNA additional purification
25 Annex C (informative) Repeatability and reproducibility
29 Annex D (informative) Practical application to various textile products
30 Bibliography

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BS ISO 18074:2015
$167.15