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BS EN ISO 20743:2021 – TC

BS EN ISO 20743:2021 – TC

$217.84

Tracked Changes. Textiles. Determination of antibacterial activity of textile products

Published By Publication Date Number of Pages
BSI 2021 88
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This document specifies quantitative test methods to determine the antibacterial activity of all antibacterial textile products including nonwovens.

This document is applicable to all textile products, including cloth, wadding, thread and material for clothing, bedclothes, home furnishings and miscellaneous goods, regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting).

This document covers three inoculation methods for the determination of antibacterial activity:

  1. absorption method (an evaluation method in which the test bacterial suspension is inoculated directly onto specimens);

  2. transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto specimens);

  3. printing method (an evaluation method in which test bacteria are placed on a filter and printed onto specimens).

NOTE

Based on the intended application and on the environment in which the textile product is to be used, and also on the surface properties of the textile properties, the user can select the most suitable inoculation method.

This document also specifies the colony plate count method and the adenosine triphosphate (ATP) luminescence method for measuring the enumeration of bacteria.

PDF Catalog

PDF Pages PDF Title
48 National foreword
50 European foreword
53 Foreword
54 Introduction
55 1 Scope
2 Normative references
3 Terms and definitions
56 4 Safety precaution
5 Apparatus
58 5.26 Aluminium foil.
5.27 Reciprocal incubation shaker.
6 Reagents and culture media
6.1 Water
6.2 Tryptone soya broth (TSB)
6.3 Tryptone soya agar (TSA)
59 6.4 Agar for transfer
6.5 Nutrient broth (NB)
6.6 Peptone salt solution
6.7 Physiological saline
6.8 Soybean-Casein Digest Broth with Lecithin & Polysorbate 80 (SCDLP) medium
60 6.9 Dilution buffer for shake-out bacterial suspension
6.10 Neutralizing solution
6.11 Enumeration agar (EA)
61 6.12 Agar for printing
6.13 Cryoprotective solution for bacterial species
6.14 Stock solution of ATP standard reagent
6.15 Buffer solution for ATP luminescent reagent
62 6.16 ATP luminescent reagent
6.17 ATP extracting reagent
6.18 ATP eliminating reagent
63 6.19 SCDLP or other medium for preparing ATP reference solution
6.20 Shake-out physiological saline
7 Reference strains
7.1 Strains
7.2 Restoration and storage of strains
7.2.1 General
7.2.2 Ceramic bead method
64 7.2.3 Glycerol suspension method
8 Test procedures
8.1 Absorption method (see Annex E)
8.1.1 Incubation of test strain
65 8.1.2 Preparation of test inoculum
8.1.3 Preparation of test specimens
66 8.1.4 Test operation
67 8.1.5 Test results
68 8.2 Transfer method (see Annex E)
8.2.1 Preparation of test inoculum
69 8.2.2 Preparation of specimens
8.2.3 Test operation
70 8.2.4 Test results
71 8.3 Printing method (see Annex E)
8.3.1 Incubation of test strain
72 8.3.2 Preparation of test inoculum
8.3.3 Pretreatment of specimen
8.3.4 Test operation
75 8.3.5 Test results
9 Judgement of antibacterial efficacy
10 Test report
77 Annex A (normative) Strain numbers
78 Annex B (normative) Shaking method
79 Annex C (normative) Quantitative measurement by plate count method
80 Annex D (normative) Quantitative measurement by luminescence method
82 Annex E (informative) Testing examples
85 Annex F (informative) Efficacy of antibacterial activity
86 Bibliography

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BS EN ISO 20743:2021 - TC
$217.84