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BS EN ISO 10272-2:2017

BS EN ISO 10272-2:2017

$142.49

Microbiology of the food chain. Horizontal method for detection and enumeration of Campylobacter spp. – Colony-count technique

Published By Publication Date Number of Pages
BSI 2017 28
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This document specifies a horizontal method for the enumeration of Campylobacter spp. It is applicable to

  • products intended for human consumption,

  • products intended for animal feeding,

  • environmental samples in the area of food and feed production, handling, and

  • samples from the primary production stage such as animal faeces, dust, and swabs.

PDF Catalog

PDF Pages PDF Title
2 National foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
4.1 General
4.2 Preparation of dilutions
4.3 Enumeration
4.4 Confirmation
5 Culture media and reagents
11 6 Equipment and consumables
7 Sampling
8 Preparation of test sample
12 9 Procedure
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation and incubation
9.3 Enumeration of characteristic colonies
9.4 Confirmation of Campylobacter
9.4.1 General
13 9.4.2 Selection of colonies for confirmation
9.4.3 Examination of morphology and motility
9.4.4 Study of aerobic growth at 25 °C
9.4.5 Detection of oxidase activity
9.4.6 Interpretation
14 9.5 Identification of Campylobacter species (optional)
9.5.1 General
9.5.2 Detection of catalase activity
9.5.3 Detection of hippurate hydrolysis
9.5.4 Detection of indoxyl acetate hydrolysis
15 9.5.5 Interpretation
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory study
11.2 Repeatability limit
16 11.3 Reproducibility limit
17 12 Test report
18 Annex A (normative) Diagram of procedure
19 Annex B (normative) Culture media and reagents
24 Annex C (informative) Method validation studies and performance characteristics
27 Bibliography

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BS EN ISO 10272-2:2017
$142.49