BS EN 17250:2020

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Foodstuffs. Determination of ochratoxin A in spices, liquorice, cocoa and cocoa products by IAC clean-up and HPLC-FLD

Published By	Publication Date	Number of Pages
BSI	2020	30

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Categories: 67.220.10 - Spices and condiments, BSI

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Description

This document specifies a procedure for the determination of ochratoxin A (OTA) in chilli, paprika, black and white pepper, nutmeg, spice mix, liquorice (root and extracts), cocoa and cocoa products by high performance liquid chromatography (HPLC) with immunoaffinity column clean-up and fluorescence detection (FLD).

This method has been validated in interlaboratory studies via the analysis of both naturally contaminated and spiked samples ranging from 1,0 ?g/kg to 84,9 ?g/kg for spices (paprika and chili [5], black and white pepper, nutmeg and spice mix [6]), ranging from 7,7 ?g/kg to 96,8 ?g/kg for liquorice and liquorice products [7] and ranging from 2,1 ?g/kg to 26,3 ?g/kg for cocoa and cocoa products [6].

For further information on the validation, see Clause 10 and Annex B.

PDF Catalog

PDF Pages	PDF Title
2	undefined
7	1 Scope 2 Normative references 3 Terms and definitions 4 Principle 5 Reagents
11	6 Apparatus and equipment
12	7 Procedure 7.1 Preparation of the test sample 7.2 Extraction 7.3 Immunoaffinity column clean-up 7.4 Preparation of sample test solutions
13	7.5 Spiking procedure (optional, if no certified reference material (CRM) is used) 8 HPLC analysis 8.1 HPLC operating conditions 8.1.1 General 8.1.2 Paprika and chilli (mobile phase A)
14	8.1.3 Liquorice and liquorice products (mobile phase B)
15	8.1.4 Spices, cocoa and cocoa products (mobile phase C) 8.2 Calibration curve 8.3 Identification 9 Calculation
16	10 Precision 10.1 General 10.2 Repeatability 10.3 Reproducibility
18	11 Test report
19	Annex A (informative)Typical chromatograms
23	Annex B (informative)Precision data

Additional information

Status	Definitive
Pages	30
Publication Date	2020-01-30
ISBN	978 0 580 98502 7
Standard Number	BS EN 17250:2020
Title	Foodstuffs. Determination of ochratoxin A in spices, liquorice, cocoa and cocoa products by IAC clean-up and HPLC-FLD
Identical National Standard Of	EN EN
Descriptors	Food, Ochratoxin A, Liquorice (plant), Cocoa beans, Products
Publisher	BSI
Committee	AW/275
ICS Codes	67.220.10 - Spices and condiments

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BS EN 17250:2020

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