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BS EN 14152:2014

BS EN 14152:2014

$102.76

Foodstuffs. Determination of vitamin B2 by high performance liquid chromatography

Published By Publication Date Number of Pages
BSI 2014 20
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This European Standard specifies a method for the determination of vitamin B2 in food by high performance liquid chromatography (HPLC) and fluorescence detection. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of milk powder and pig’s liver ranging from 1,45 mg/100 g to 10,68 mg/100 g. The second study was for the analysis of samples of tube feeding solution, baby food, powdered milk, meal with fruits, yeast, cereal and chocolate powder ranging from 0,21 mg/100 g to 87,1 mg/100 g. Vitamin B2 is the mass fraction of total riboflavin including its phosphorylated derivatives.

For further information on the validation, see Clause 8 and Annex B.

PDF Catalog

PDF Pages PDF Title
5 Foreword
6 1 Scope
2 Normative references
3 Principle
4 Reagents
8 5 Apparatus
9 6 Procedure
6.1 Precautions
6.2 Preparation of the test sample
6.3 Preparation of the sample test solution
6.3.1 Extraction
6.3.2 Enzyme treatment
10 6.3.3 Sample test solution
6.4 Identification
6.5 Determination
7 Calculation
11 8 Precision
8.1 General
8.2 Repeatability
12 8.3 Reproducibility
9 Test report
13 Annex A (informative) Examples of HPLC chromatograms
Figure A.1 — Example for a HPLC separation of riboflavin standard solution a) and infant formula b)
14 Annex B (informative) Precision data
Table B.1 — Precision data for milk powder and pig liver
15 Table B.2 — Precision data for tube feeding solution, baby food with vegetables, powdered milk, meal with fruits and yeast
Table B.3 — Precision data for cereal, chocolate powder and food supplement
16 Annex C (informative) Alternatives HPLC systems
Table C.1 – Alternative HPLC conditions
17 Bibliography

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BS EN 14152:2014
$102.76