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BS EN 13697:2023 – TC

$217.84

Tracked Changes. Chemical disinfectants and antiseptics. Quantitative non-porous surface test for the evaluation of bactericidal and yeasticidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas without mechanical action. Test method and requirements without mechanical action (phase 2, step 2)

Published By Publication Date Number of Pages
BSI 2023 91
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This document specifies a test method (phase 2/step 2) and the minimum requirements for bactericidal and yeasticidal and/or fungicidal activity of chemical disinfectants that form a homogeneous physically stable preparation in hard water or – in the case of ready-to-use products – with water in food, industrial, domestic and institutional areas, excluding areas and situations where disinfection is medically indicated and excluding products used on living tissues. This document applies to products that are used for disinfecting non-porous surfaces without mechanical action. This document is applicable at least to the following: a) processing, distribution and retailing of: 1) food of animal origin: i) milk and milk products; ii) meat and meat products; iii) fish, seafood and products; iv) eggs and egg products; v) animal feeds; vi) etc. 2) food of vegetable origin: i) beverages; ii) fruits, vegetables and derivatives (including distilleries and sugar refineries); iii) flour, milling and baking; iv) animal feeds; v) etc. b) institutional and domestic areas: 1) catering establishments; 2) public areas; 3) public transports; 4) schools; 5) nurseries; 6) shops; 7) sports rooms; 8) waste containers (bins); 9) hotels; 10) dwellings; 11) clinically non sensitive areas of hospitals; 12) offices; 13) etc. c) other industrial areas: 1) packaging material; 2) biotechnology (yeast, proteins, enzymes…); 3) pharmaceutical; 4) cosmetics and toiletries; 5) textiles; 6) space industry, computer industry; 7) etc. This document does not apply when the product is applied via an automatic airborne disinfection method; in such cases, see EN 17272. Using this document, it is possible to determine the bactericidal and/or yeasticidal and/or fungicidal activity of the undiluted product. As three concentrations are tested, in the active to non-active range, dilution of the product is required and, therefore, the product forms a homogeneous stable preparation in hard water. EN 14885 specifies in detail the relationship of the various tests to one another and to use recommendations. NOTE 1 The method described is intended to determine the activity of commercial formulations or active substances on bacteria and/or fungi in the conditions in which they are used. NOTE 2 This method cannot be used to evaluate the activity of products against mycobacteria.

PDF Catalog

PDF Pages PDF Title
1 30482684
47 A-30457013
48 undefined
54 1 Scope
55 2 Normative references
56 3 Terms and definitions
4 Requirements
58 5 Test methods
5.1 Principle
5.2 Materials and reagents
5.2.1 Test organisms
59 5.2.2 Culture media and reagents
5.2.2.1 General
5.2.2.2 Water
5.2.2.3 Tryptone Soya Agar (TSA)
5.2.2.4 Malt extract agar (MEA)
60 5.2.2.5 Diluent
5.2.2.6 Neutralizer
5.2.2.7 Hard water for dilution of the products
61 5.2.2.8 Interfering substances
62 5.2.3 Test surface
5.3 Apparatus and glassware
63 5.4 Preparation of test organism suspensions and product test solutions
5.4.1 Test organism suspensions
5.4.1.1 Stock cultures of test organisms
64 5.4.1.2 Working culture of test organisms
5.4.1.3 Test suspensions
66 5.4.1.4 Quantifying of bacterial and fungal test suspensions
5.4.1.5 Calculating the weighed mean of the test suspensions
67 5.4.2 Product test solutions
5.5 Procedure
5.5.1 Choice of experimental conditions
68 5.5.2 Test procedure
5.5.2.1 Test “Na” – determination of microbicidal concentrations
71 5.5.2.2 Water control “A”
5.5.2.3 Neutralizer control “B” – verification of the absence of toxicity of the neutralizer
72 5.5.2.4 Method validation “C” – dilution-neutralization validation
5.5.3 Quantifying of the test mixtures
73 5.6 Calculation and expression of results
5.6.1 Elaboration of data: counting of weighed average values
74 5.6.2 Verification of methodology
5.6.3 Expression of results
5.6.4 Conclusion
5.6.4.1 Activity on non-porous surfaces for general purposes
75 5.6.4.2 Activity on non-porous surfaces for dairies
5.6.4.3 Activity on non-porous surfaces for specific purposes
5.7 Test report
78 Annex A (informative)Corresponding referenced strains
80 Annex B (informative)Neutralizers
82 Annex C (informative)Expression of results with the dilution-neutralization method
84 Annex D (informative)Bactericidal activity in surfaces in general use conditions (for clean conditions)
86 Annex E (informative)Precision of the test result
87 Annex F (informative)Alternative drying end point
BS EN 13697:2023 - TC
$217.84