{"id":338322,"date":"2024-10-19T23:40:19","date_gmt":"2024-10-19T23:40:19","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-169242017\/"},"modified":"2024-10-25T22:46:25","modified_gmt":"2024-10-25T22:46:25","slug":"bs-en-169242017","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-169242017\/","title":{"rendered":"BS EN 16924:2017"},"content":{"rendered":"
This European Standard describes a procedure for the determination of the zearalenone content in edible vegetable oils specifically maize germ oil by either of the following techniques: High performance liquid chromatography with fluorescence detection (LC-FLD) or high performance liquid chromatography with tandem mass spectrometry (LC-MS\/MS) after basic extraction of the diluted oil. The method has been validated for zearalenone in naturally contaminated maize germ oil at levels of 61,2 \u00b5g\/kg to 515 \u00b5g\/kg [5]. Laboratory experiences [6] have shown that this method is also applicable to other vegetable oils such as wheat germ oil (n = 4), sunflower oil (n = 5), pumpkin seed oil (n = 1), soybean oil (n = 5), hemp seed oil (n = 5), rape seed oil (n = 11), and mixed oils including maize germ oil (n = 3). However occasionally, samples can result in interferences in the FLD-chromatograms. In this case, the detection with MS\/MS is recommended.<\/p>\n
PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
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2<\/td>\n | National foreword <\/td>\n<\/tr>\n | ||||||
7<\/td>\n | 1 Scope 2 Normative references 3 Principle 4 Reagents <\/td>\n<\/tr>\n | ||||||
10<\/td>\n | 5 Apparatus and equipment <\/td>\n<\/tr>\n | ||||||
12<\/td>\n | 6 Procedure 6.1 Extraction of zearalenone from the sample 6.1.1 General 6.1.2 Preparation for LC-FLD 6.1.3 Preparation for LC-MS\/MS 6.2 LC-FLD analysis <\/td>\n<\/tr>\n | ||||||
13<\/td>\n | 6.3 LC-MS\/MS analysis <\/td>\n<\/tr>\n | ||||||
14<\/td>\n | 6.4 Identification 7 Calculation 7.1 Calculation with external standard (FLD measurement) <\/td>\n<\/tr>\n | ||||||
15<\/td>\n | 7.2 Calculation with external standard (LC-MS\/MS measurement) 7.3 Calculation with the internal standard zearalanone (ISTD\u00a01) <\/td>\n<\/tr>\n | ||||||
16<\/td>\n | 8 Precision 8.1 General 8.2 Repeatability 8.3 Reproducibility <\/td>\n<\/tr>\n | ||||||
17<\/td>\n | 9 Test report <\/td>\n<\/tr>\n | ||||||
18<\/td>\n | Annex\u00a0A (informative)Typical chromatograms A.1 Example chromatograms for LC-FLD method <\/td>\n<\/tr>\n | ||||||
19<\/td>\n | A.2 Example chromatograms for LC-MS\/MS method <\/td>\n<\/tr>\n | ||||||
24<\/td>\n | Annex\u00a0B (informative)Example conditions for suitable LC-MS\/MS systems B.1 System settings for Waters Quattro Premier B.1.1 Settings for chromatography B.1.2 Detector parameters <\/td>\n<\/tr>\n | ||||||
25<\/td>\n | B.2 System settings for AB Sciex API\u00a03000\u2122) B.2.1 Settings for chromatography B.2.2 Detector parameters <\/td>\n<\/tr>\n | ||||||
26<\/td>\n | B.3 Detector parameters for AB Sciex API\u00a04000\u2122) <\/td>\n<\/tr>\n | ||||||
27<\/td>\n | B.4 Detector parameters for Sciex Triple Quad\u2122 5500 System) <\/td>\n<\/tr>\n | ||||||
29<\/td>\n | Annex\u00a0C (informative)Precision data <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Foodstuffs. Determination of zearalenone in edible vegetable oils by LC-FLD or LC-MS\/MS<\/b><\/p>\n |