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BS EN 16924:2017

BS EN 16924:2017

$167.15

Foodstuffs. Determination of zearalenone in edible vegetable oils by LC-FLD or LC-MS/MS

Published By Publication Date Number of Pages
BSI 2017 34
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This European Standard describes a procedure for the determination of the zearalenone content in edible vegetable oils specifically maize germ oil by either of the following techniques: High performance liquid chromatography with fluorescence detection (LC-FLD) or high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) after basic extraction of the diluted oil. The method has been validated for zearalenone in naturally contaminated maize germ oil at levels of 61,2 Āµg/kg to 515 Āµg/kg [5]. Laboratory experiences [6] have shown that this method is also applicable to other vegetable oils such as wheat germ oil (n = 4), sunflower oil (n = 5), pumpkin seed oil (n = 1), soybean oil (n = 5), hemp seed oil (n = 5), rape seed oil (n = 11), and mixed oils including maize germ oil (n = 3). However occasionally, samples can result in interferences in the FLD-chromatograms. In this case, the detection with MS/MS is recommended.

PDF Catalog

PDF Pages PDF Title
2 National foreword
7 1 Scope
2 Normative references
3 Principle
4 Reagents
10 5 Apparatus and equipment
12 6 Procedure
6.1 Extraction of zearalenone from the sample
6.1.1 General
6.1.2 Preparation for LC-FLD
6.1.3 Preparation for LC-MS/MS
6.2 LC-FLD analysis
13 6.3 LC-MS/MS analysis
14 6.4 Identification
7 Calculation
7.1 Calculation with external standard (FLD measurement)
15 7.2 Calculation with external standard (LC-MS/MS measurement)
7.3 Calculation with the internal standard zearalanone (ISTDĀ 1)
16 8 Precision
8.1 General
8.2 Repeatability
8.3 Reproducibility
17 9 Test report
18 AnnexĀ A (informative)Typical chromatograms
A.1 Example chromatograms for LC-FLD method
19 A.2 Example chromatograms for LC-MS/MS method
24 AnnexĀ B (informative)Example conditions for suitable LC-MS/MS systems
B.1 System settings for Waters Quattro Premier
B.1.1 Settings for chromatography
B.1.2 Detector parameters
25 B.2 System settings for AB Sciex APIĀ 3000ā„¢)
B.2.1 Settings for chromatography
B.2.2 Detector parameters
26 B.3 Detector parameters for AB Sciex APIĀ 4000ā„¢)
27 B.4 Detector parameters for Sciex Triple Quadā„¢ 5500 System)
29 AnnexĀ C (informative)Precision data

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BS EN 16924:2017
$167.15